Antioxidant and α; ylase inhibitory property of phyllanthus virgatus L.: An in vitro and molecular interaction study

26Citations
Citations of this article
54Readers
Mendeley users who have this article in their library.

Abstract

The present study on Phyllanthus virgatus , known traditionally for its remedial potential, for the first time provides descriptions of the antioxidant and inhibition of α -amylase enzyme activity first by in vitro analyses, followed by a confirmatory in silico study to create a stronger biochemical rationale. Our results illustrated that P. virgatus methanol extract exhibited strong antioxidant and oxidative DNA damage protective activity than other extracts, which was well correlated with its total phenolic content. In addition, P. virgatus methanol extract strongly inhibited the α -amylase activity (IC 50 33.20 ± 0.556 μ g/mL), in a noncompetitive manner, than acarbose (IC 50 76.88 ± 0.277 μ g/mL), which showed competitive inhibition. Moreover, this extract stimulated the glucose uptake activity in 3T3-L1 cells and also showed a good correlation between antioxidant and α -amylase activities. The molecular docking studies of the major bioactive compounds (9,12-octadecadienoic acid, asarone, 11-octadecenoic acid, and acrylic acid) revealed via GC-MS analysis from this extract mechanistically suggested that the inhibitory property may be due to the synergistic effect of these bioactive compounds. These results provide substantial basis for the future use of P. virgatus methanol extract and its bioactive compound in in vivo system for the treatment and management of diabetes as well as in the related condition of oxidative stress.

Cite

CITATION STYLE

APA

Hashim, A., Khan, M. S., Khan, M. S., Baig, M. H., & Ahmad, S. (2013). Antioxidant and α; ylase inhibitory property of phyllanthus virgatus L.: An in vitro and molecular interaction study. BioMed Research International, 2013. https://doi.org/10.1155/2013/729393

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free