It is known that SPARC gates VEGF-A signal transduction towards KDR, the primary angiogenic VEGF receptor. We sought to determine whether inhibition of SPARC activity using anti-SPARC peptide could inhibit laser-induced CNV by promoting binding of VEGF-A to FLT-1. We created anti-SPARC l-peptide and retro-inverso anti-SPARC d-peptide. Anti-SPARC peptides or PBS were injected intravitreally 1. day before or after laser induction. Intravitreal injection of anti-SPARC l-peptide 1. day before laser induction promotes FLT-1 phosphorylation and inhibited laser-induced CNV and anti-SPARC d-peptide had no effect. Injection 1. day after laser injury did not affect size of laser-induced CNV. Inhibition of SPARC activity could be complementary to existing anti-CNV therapy. © 2009 Elsevier Ltd.
Uehara, H., Luo, L., Simonis, J., Singh, N., Taylor, E. W., & Ambati, B. K. (2010). Anti-SPARC oligopeptide inhibits laser-induced CNV in mice. Vision Research, 50(7), 674–679. https://doi.org/10.1016/j.visres.2009.12.003