Antiviral activity of derivatized dextrans on HIV-1 infection of primary macrophages and blood lymphocytes

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Abstract

The present study demonstrates at the molecular level that dextran derivatives carboxymethyl dextran benzylamine (CMDB) and carboxymethyl dextran benzylamine sulfonate (CMDBS), characterized by a statistical distribution of anionic carboxylic groups, hydrophobic benzylamide units, and/or sulfonate moieties, interact with HIV-1 LAI gp120 and V3 consensus clades B domain. Only limited interaction was observed with carboxy-methyl dextran (CMD) or dextran (D) under the same conditions. CMDBS and CMDB (1 μM) strongly inhibited HIV-1 infection of primary macrophages and primary CD4+ lymphocytes by macrophage-tropic and T lymphocyte-tropic strains, respectively, while D or CMD had more limited effects on M-tropic infection of primary macrophages and exert no inhibitory effect on M- or T-tropic infection of primary lymphocytes. CMDBS and CMDB (1 μM) had limited but significant effect on oligomerized soluble recombinant gp120 binding to primary macrophages while they clearly inhibit (> 50%) such binding to primary lymphocytes. In conclusion, the inhibitory effect of CMDB and the CMDBS, is observed for HIV M- and T-tropic strain infections of primary lymphocytes and macrophages which indicates that these compounds interfere with steps of HIV replicative cycle which neither depend on the virus nor on the cell.

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Seddiki, N., Mbemba, E., Letourneur, D., Ylisastigui, L., Benjouad, A., Saffar, L., … Gattegno, L. (1997). Antiviral activity of derivatized dextrans on HIV-1 infection of primary macrophages and blood lymphocytes. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1362(1), 47–55. https://doi.org/10.1016/S0925-4439(97)00057-4

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