Application of UV-Spectrophotometry and RP-HPLC for Simultaneous Determination of Atorvastatin Calcium and Ezetimibe in Pharmaceutical Dosage Form

  • Sonawane S
  • Shirkhedkar A
  • Fursule R
  • et al.
N/ACitations
Citations of this article
16Readers
Mendeley users who have this article in their library.

Abstract

Two methods are described for the simultaneous determination of Atorvastatin calcium and Ezetimibe in binary mixture. The first method was based on UV-spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 232.5 nm (λmax of Ezetimibe) and 246.0 nm (λmax of Atorvastatin calcium) in methanol; linearity was obtained in the range of 5 – 25 μg.mL-1 for both the drugs. The second method was based on HPLC separation of the two drugs in reverse phase mode using Luna C18 column. Linearity was obtained in the concentration range of 8-22 μg.mL-1 for both the drugs. Both these methods have been successively applied to pharmaceutical formulation and were validated according to ICH guidelines.

Cite

CITATION STYLE

APA

Sonawane, S., Shirkhedkar, A., Fursule, R., & Surana, S. (2017). Application of UV-Spectrophotometry and RP-HPLC for Simultaneous Determination of Atorvastatin Calcium and Ezetimibe in Pharmaceutical Dosage Form. Eurasian Journal of Analytical Chemistry, 1(1), 31–41. https://doi.org/10.12973/ejac/77004

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free