Association of glucose-regulated protein (grp78) with human keratin 8

13Citations
Citations of this article
7Readers
Mendeley users who have this article in their library.

Abstract

Keratin polypeptides 8 and 18 (K8/18) are intermediate filament proteins that are expressed in 'simple-type' epithelial cells. They associate with several proteins including the 70 kDa cytoplasmic heatshock proteins (Hsp70). We identified the human 78 kDa glucose-regulated protein (grp78) as a keratin-associated protein. Keratin-grp78 association was noted after co-immunoprecipitation Of K8/18 from HT29 detergent solubilized cell lysates, and appears to involve non-posttranslationally modified grp78. The grp78-K8/18 association is induced by culturing cells in the presence of tunicamycin or after glucose starvation, K8/18-bound grp78 can be dissociated by Mg-ATP and the association can be reconstituted in vitro using purified grp78, then redissociated again by Mg-ATP. Binding of grp78 occurs preferentially with K8, and when reconstituted does not depend on the posttranslational modification state of K8/18. Coincubation of K8/18 with hsp70 and grp78 shows preferential association with hsp70. Our results demonstrate a direct association of grp78 with K8 under conditions that induce grp78 expression.

Cite

CITATION STYLE

APA

Liao, J., Price, D., & Omary, M. B. (1997). Association of glucose-regulated protein (grp78) with human keratin 8. FEBS Letters, 417(3), 316–320. https://doi.org/10.1016/S0014-5793(97)01315-X

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free