This study aimed at investigating the association between haemostatic biomarkers, proinflammatory, and anti-inflammatory cytokines with chronic kidney disease in type 1 diabetic patients. Patients were divided into two groups: with nephropathy (albuminuria ≥ 30 mg/g and/or GFR < 60 mL/min/1.73 m 2), í µí± = 65; and without nephropathy (albuminuria < 30 mg/g and GFR ≥ 60 mL/min/1.73 m 2), í µí± = 60. INF-í µí»¾, IL-6, IL-10, and TNF-í µí»¼ plasma levels were determined by flow cytometry. VWF, ADAMTS13 antigen, and D-Dimer plasma levels were determined by enzyme-linked immunosorbent assay and ADAMTS13 activity was assessed by fluorescence resonance energy transfer assay. Elevated levels of INF-í µí»¾, VWF, ADAMTS13 antigen, D-Dimer, and reduced ADAMTS13 activity/antigen ratio were observed in patients with nephropathy as compared to those without nephropathy (í µí± = 0.001, í µí± < 0.001, í µí± < 0.001, í µí± < 0.001, and í µí± < 0.001, resp.). Cytokines and haemostatic biomarkers remained associated with nephropathy after adjustments (use of statin, acetylsalicylic acid, angiotensin converting enzyme inhibitor, and angiotensin antagonist). INF-í µí»¾, TNF-í µí»¼, and IL-10 significantly correlated with haemostatic biomarkers. Inflammatory and hypercoagulability status are associated with nephropathy in type 1 diabetes mellitus and an interrelationship between them may play an important role in pathogenesis of diabetic nephropathy.
Pereira Domingueti, C., Bastos Fóscolo, R., Sepúlveda Reis, J., Magalhães Freire Campos, F., Maria Dusse, L. S., das Graças Carvalho, M., … Paula Fernandes, A. (2016). Association of Haemostatic and Inflammatory Biomarkers with Nephropathy in Type 1 Diabetes Mellitus. Journal of Diabetes Research, 2016. https://doi.org/10.1155/2016/2315260