Asterless Is a Centriolar Protein Required for Centrosome Function and Embryo Development in Drosophila

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Abstract

Background: Centrosomes, the major organizers of the microtubule network in most animal cells, are composed of centrioles embedded in a web of pericentriolar material (PCM). Recruitment and stabilization of PCM on the centrosome is a centriole-dependent function. Compared to the considerable number of PCM proteins known, the molecular characterization of centrioles is still very limited. Only a few centriolar proteins have been identified so far in Drosophila, most related to centriole duplication. Results: We have cloned asterless (asl) and found that it encodes a 120 kD highly coiled-coil protein that is a constitutive pancentriolar and basal body component. Loss of asl function impedes the stabilization/maintenance of PCM at the centrosome. In embryos deficient for Asl, development is arrested right after fertilization. Asl shares significant homology with Cep152, a protein described as a component of the human centrosome for which no functional data is yet available. Conclusions: The cloning of asl offers new insight into the molecular composition of Drosophila centrioles and a possible model for the role of its human homolog. In addition, the phenotype of asl-deficient flies reveals that a functional centrosome is required for Drosophila embryo development. © 2007 Elsevier Ltd. All rights reserved.

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Varmark, H., Llamazares, S., Rebollo, E., Lange, B., Reina, J., Schwarz, H., & Gonzalez, C. (2007). Asterless Is a Centriolar Protein Required for Centrosome Function and Embryo Development in Drosophila. Current Biology, 17(20), 1735–1745. https://doi.org/10.1016/j.cub.2007.09.031

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