The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers (MIPs; known as `smart materials') for inducing protein crystal growth. Modified MIPs of altered texture suitable for high-throughput trials are demonstrated to improve crystal quality and to increase the probability of success when screening for suitable crystallization conditions. The application of these materials is simple, time-efficient and will provide a potent tool for structural biologists embarking on crystallization trials.
Khurshid, S., Govada, L., EL-Sharif, H. F., Reddy, S. M., & Chayen, N. E. (2015). Automating the application of smart materials for protein crystallization. Acta Crystallographica Section D Biological Crystallography, 71(3), 534–540. https://doi.org/10.1107/s1399004714027643