Metabolite localization in living drosophila using high resolution magic angle spinning NMR

Abstract

We have developed new methods enabling in vivo localization and identification of metabolites through their 1 H NMR signatures, in a drosophila. Metabolic profiles in localized regions were obtained using HR-MAS Slice Localized Spectroscopy and Chemical Shift Imaging at high magnetic fields. These methods enabled measurement of metabolite contents in anatomic regions of the fly, demonstrated by a decrease in Î 2-alanine signals in the thorax of flies showing muscle degeneration.