Functional expression of hMYH, a human homolog of the Escherichia coli MutY protein

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Abstract

We have previously described the hMYH cDNA and genomic clones (M. M. Slupska et al., J. Bacteriol. 178:3885-3892, 1996). Here, we report that the enzyme expressed from an hMYH cDNA clone in Escherichia coli complements the mutator phenotype in a mutY mutant and can remove A from an A · 8- hydroxydeoxyguanine mismatch and to a lesser extent can remove A from an A · G mismatch in vitro.

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Slupska, M. M., Luther, W. M., Chiang, J. H., Yang, H., & Miller, J. H. (1999). Functional expression of hMYH, a human homolog of the Escherichia coli MutY protein. Journal of Bacteriology, 181(19), 6210–6213. https://doi.org/10.1128/jb.181.19.6210-6213.1999

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