Effect of Plant Growth Regulators and Liquid-Solid Nature of the Media on In-vitro Propagation of Curcuma longa (Turmeric)

Abstract

Micropropagation of Curcuma longa is important for obtaining large scale disease-free planting materials for year-round cultivation, conservation of germplasm and crop improvement programs. The present study was conducted to study the effect of different hormonal combination and solid liquid nature of the media on successful in-vitro regeneration of explants. The results revealed that Murashige and Skoog (MS) medium supplemented with 4.00 mg l-1 Benzyl amino purine (BAP) and 0.25 mgl-1 Naphthalene acetic acid (NAA) has shown the best performances in multiplication. Culturing in liquid media followed by solid culture media was more effective for shoot growth and multiplication compared to continuous culturing in solid or liquid media during the multiplication cycle.