Protein kinase PKR catalytic activity is required for the PKR-dependent activation of mitogen-activated protein kinases and amplification of interferon beta induction following virus infection

21Citations
Citations of this article
22Readers
Mendeley users who have this article in their library.

Abstract

The protein kinase regulated by RNA (PKR) enhances both activation of mitogen-activated protein kinases and the induction of interferon beta (IFN-β) by measles virus defective in C-protein expression (C ko). Here we used complementation of human cell lines stably deficient in PKR (PKR kd) to probe the basis of these PKR-mediated responses. We found that PKR kd HeLa and amnion U cell lines were defective for virus-mediated activation of IFN induction signaling components compared to PKR-sufficient control cells. Complementation of PKR kd cells with wildtype PKR, but not with PKR mutants defective in either catalytic activity or dsRNA-binding activity, restored JNK, p38 and ATF-2 phosphorylation and enhanced IFN-β induction following infection. By contrast to mammalian PKR, the Z-DNA binding domain-containing fish homologue of PKR, PKZ, lacked the capacity to enhance C ko virus-mediated IFN-β induction. Furthermore, inhibition of virus growth was observed with C ko-infected PKR kd cells complemented with PKR but not with PKZ. © 2012 Elsevier Inc.

Cite

CITATION STYLE

APA

Taghavi, N., & Samuel, C. E. (2012). Protein kinase PKR catalytic activity is required for the PKR-dependent activation of mitogen-activated protein kinases and amplification of interferon beta induction following virus infection. Virology, 427(2), 208–216. https://doi.org/10.1016/j.virol.2012.01.029

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free