The role of members of the mitogen-activated protein kinase (MAPK) family on tumor necrosis factor α (TNF-α)-mediated down-regulation of col1a1 gene was studied. TNF-α increased extracellular-regulated kinase and Jun-N-terminal kinase phosphorylation, but these effects were not related to its inhibitory effect on α1(I) procollagen (col1a1) mRNA levels. Phosphorylation of p38 MAPK was decreased in response to TNF-α, and the specific p38 MAPK inhibitor SB203580 mimicked the effect of TNF-α on col1a1 mRNA levels. Transforming growth factor β (TGF-β) increased p38 MAPK phosphorylation and SB203580 prevented the induction of col1a1 mRNA levels by TGF-β. These results suggest that p38 MAPK plays an important role in regulating the expression of col1a1 in hepatic stellate cells in response to cytokines. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Varela-Rey, M., Montiel-Duarte, C., Osés-Prieto, J. A., López-Zabalza, M. J., Jaffrèzou, J. P., Rojkind, M., & Iraburu, M. J. (2002). p38 MAPK mediates the regulation of α1(I) procollagen mRNA levels by TNF-α and TGF-β in a cell line of rat hepatic stellate cells. FEBS Letters, 528(1–3), 133–138. https://doi.org/10.1016/S0014-5793(02)03276-3