Expression of nitrite transporter gene to increase nitrate absoprtion in rice plant (Oryza sativa Cv nipponbare) as sole nitrogen source

Abstract

Incorporated of CsNitr1-L-transgene in rice plant was showed to active and transport NO2- from cytosol into chloroplast. With a PCR method screening of CsNitr1-L gene were confirmed which the CsNitr1-L-transgene have amplification band, while the plant were not contained CsNitr1-L gene, there are no amplification band. Some strain were obtained and got a seeds. Almost CsNitr1-L-transgene was raising in all parameter test, such as plant height, tillers number, respectively. The accumulation of NO2- in the leaves of CsNitr1-L-transgene was lower compared with wild type. CsNitr1-L is a cucumber NO2- -transporter gene that located in chloroplast membrane have been transformed into japonica rice cv. Nipponbare. A series experiments was carried out to understand the expression- CsNitr1-L-transgenic rice. The seeds were cultivated to observe the activity of CsNitr1-L-transgene with medium containing NO3- or NO2- as inducer. Agronomical test, like tillers number and plant height were observed and compare with non CsNitr1-L- transgenic rice. While the NO2- content in leaves were analyzed with capillary electrophoresis method.