Correlation between p16 and Interleukin-2 Gene Expression and Oral and Maxillofacial Wound Healing

Abstract

To analyze the relationship between p16 and interleukin-2 protein and messenger RNA levels and wound healing in patients with oral and maxillofacial trauma. 86 patients with oral and maxillofacial trauma were selected in our hospital from June 2018 to June 2020 and divided into good and poor trauma healing group. All patients were treated with vacuum sealing drainage and given empirical antibacterial anti-infective medication, nutritional support and correction of fluid-electrolyte disturbances for 7 d. Serum levels of inflammatory factors were measured before and after 7 d of treatment. The levels of interleukin-2, p16 and messenger RNA were measured by western blot and polymerase chain reaction. White blood cells, tumor necrosis factor alpha, interleukin-6, C-reactive protein and procalcitonin were lower in both groups than before treatment while the phosphorylated form of p16/p16, phosphorylated form of interleukin-2/ interleukin-2 proteins and messenger RNA levels were lower immediately after extubation (p<0.05). Pearson correlation analysis revealed that phosphorylated form of p16/p16 and phosphorylated form of interleukin-2/ interleukin-2 were significantly lower in the group immediately after extubation (p<0.05). Phosphorylated form of p16/p16 and phosphorylated form of interleukin-2/interleukin-2 were positively correlated with white blood cells, tumor necrosis factor alpha, interleukin-6, C-reactive protein and procalcitonin after treatment (p<0.001). It is concluded that p16 and interleukin-2 in the drainage fluid of patients with oral and maxillofacial trauma can promote the process of wound healing by promoting the release of serum inflammatory factors.