Molecular cloning of mouse 17β-hydroxysteroid dehydrogenase type 1 and characterization of enzyme activity

Abstract

The biological activity of certain estrogens and androgens is modulated by enzymes called 17β-hydroxysteroid dehydrogenases (17β-HSDs), which catalyze the interconversion between less active 17-oxosteroid and more active 17β-hydroxysteroid forms. In the present report, we describe cloning of mouse 17β-HSD type-1 cDNA from an ovarian library generated from 4,4'-(1,2-diethyl-1,2-ethenediyl)bisphenol-(diethylstilbestrol)-treate d mice, and characterization of the corresponding enzyme. The open reading frame of the mouse 17β-HSD type-1 cDNA encodes a peptide of 344 amino acid residues with a predicted molecular mass of 36785 Da. The mouse 17β-HSD type-1 enzyme shares 63% and 93% overall identity with human and rat 17β-HSD type-1 enzymes, respectively, and the most striking differences between the mouse and human type-1 enzymes are between the amino acid residues 197 and 230 and in the carboxy terminus of the enzymes. Similarly to the human 17β-HSD type-1 enzyme, the mouse type-1 enzyme primarily catalyzes reductive reactions from 17-oxo forms to 17β-hydroxy forms in intact cultured cells, but unlike the human type-1 enzyme, the mouse enzyme does not prefer phenolic over neutral substrates. Thus, mouse 17β-HSD type 1 catalyzes reduction of androst-4-ene-3,17-dione (androstenedione) to 17β-hydroxyandrost-4-en-3-one (testosterone) as efficiently as 3β-hydroxyestra-1,3,5(10)-trien-17-one (estrone) to estra-1,3,5(10)-triene-3β,17β-diol (estradiol). 17β-HSD type 1 is predominantly expressed in mouse ovaries, in which it is located in granulosa cells.