Uji Aktivitas Antibakteri Ekstrak Buah Rivina Humilis L. terhadap Klebsiella Pneumoniae dan Escherichia Coli

Abstract

Rivina humilis L. is herbaceous bushy perennial plant found in shrubs. The fruit extract of Rivina humilis L. had experienced antibacterial activity against K. pneumoniae and E. coli using agar diffusion method. The crude extract of nonpolar fraction (n-hexane) of Rivina humilis L. showed negative antibacterial activity against K. pneumoniae and E. coli whereas the methanolic extract has antibacterial activity with 12 mm of inhibition zone for K. pneumoniae and 10 mm for E.coli in 1 mg/ml concentration. Determination of MIC (Minimum Inhibitory Concentration) was conducted with variation of concentration (mg/ml) 1; 0.8; 0.6; 0.2; 0 (methanol 96% as negative control), and chloramphenicol (positive control). The result of MIC determination against K. pneumoniae showed that the width and the inhibition zone index in the minimum concentration (0.2 mg/ml) were 7 mm and 1.36 respectively. While against E.coli, MIC determination exhibited positive result at 0.6 mg/ml with the width and the inhibition zone index were 8 mm and 1.78 respectively.