Abstract
Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.
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CITATION STYLE
Kariyama, R., Mitsuhata, R., Chow, J. W., Clewell, D. B., & Kumon, H. (2000). Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci. Journal of Clinical Microbiology, 38(8), 3092–3095. https://doi.org/10.1128/jcm.38.8.3092-3095.2000
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