NBS1 localizes to γ-H2AX foci through interaction with the FHA/BRCT domain

235Citations
Citations of this article
68Readers
Mendeley users who have this article in their library.

Abstract

DNA double-strand breaks represent the most potentially serious damage to a genome; hence, many repair proteins are recruited to nuclear damage sites by as yet poorly characterized sensor mechanisms. Here, we show that NBS1, the gene product defective in Nijmegen breakage syndrome (NBS) [1-3], physically interacts with histone, rather than damaged DNA, by direct binding to γ-H2AX. We also demonstrate that NBS1 binding can occur in the absence of interaction with hMRE11 or BRCA1. Furthermore, this NBS1 physical interaction was reduced when anti-γ-H2AX antibody was introduced into normal cells and was also delayed in AT cells, which lack the kinase activity for phosphorylation of H2AX. NBS1 has no DNA binding region but carries a combination of the fork-head associated (FHA) and the BRCA1 C-terminal domains (BRCT) [4]. We show that the FHA/BRCT domain of NBS1 is essential for this physical interaction, since NBS1 lacking this domain failed to bind to γ-H2AX in cells, and a recombinant FHA/BRCT domain alone can bind to recombinant γ-H2AX. Consequently, the FHA/BRCT domain is likely to have a crucial role for both binding to histone and for relocalization of hMRE11/hRAD50 nuclease complex to the vicinity of DNA damage.

Cite

CITATION STYLE

APA

Kobayashi, J., Tauchi, H., Sakamoto, S., Nakamura, A., Morishima, K. ichi, Matsuura, S., … Komatsu, K. (2002). NBS1 localizes to γ-H2AX foci through interaction with the FHA/BRCT domain. Current Biology, 12(21), 1846–1851. https://doi.org/10.1016/S0960-9822(02)01259-9

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free