Runx3 is a key modulator during the epithelial-mesenchymal transition of alveolar type II cells in animal models of BPD

Abstract

Bronchopulmonary dysplasia (BPD) is a major challenge for premature infants; however, the underlying mechanisms remain unclear. We previously reported that epithelial-mesenchymal transition (EMT ) in alveolar type II (AT 2) epithelial cells influences the normal alveolar development process. In this study, we wished to examine whether Runx3 is an important factor for BPD by regulating EMT in AT 2 cells. In vivo, animal models of BPD were established by placing newborn rats in hyperoxia tanks. Lung tissue and isolated AT 2 cells were collected on different days following exposure to oxygen. The pathological changes in lung tissue, alveolar development and Runx3 expression were then investigated. In vitro, RLE -6TN cells were divided into 5 groups as follows: the control, Runx3, siRunx3, transforming growth factor-ß1 (TGF-ß1) and Runx3 + TGF-ß1 groups, and the biomarkers of EMT were investigated. In the newborn rat model of BPD, Runx3 protein and mRNA levels in both lung tissue and BPD-derived AT 2 cells were significantly lower than those in the control group. The correlation between Runx3 protein expression and pulmonary development indicators was analyzed; Runx3 expression positively correlated with the radial alveolar count (RAC ) and the percentage of smooth muscle actin-positive secondary septa, but negatively correlated with alveolar wall thickness. EMT was observed in the RLE -6TN cells in which the Runx3 gene was knocked down and follwoing TGF-ß1-induced EMT stimulation; however, TGF-ß1 failed to induce EMT in the RLE -6TN cells overexpressing Runx3. On the whole, our data indicte that low Runx3 levels may promote EMT , while high Runx3 levels inhibit TGF-ß1-induced EMT . Therefore, we predict that low levels of Runx3 in BPD lung tissue may promote EMT in AT 2 cells, thus affecting alveolar development.