Alanine aminotransferase activity in serum increases significantly when serum is incubated with pyridoxal phosphate. The increase depends on the L-alanine concentration in the final assay mixture, being greatest at 800 mmol/liter. Preincubation of 22 normal sera, in a 10:1 ratio with an 8.09 mmol/liter pyridoxal phosphate solution, resulted in an increase in the alanine aminotransferase activity from 10.5 ± 4.9 U/liter (mean ± SD) to 28.4 ± 5.3 U/liter, an increase of 170%. The absolute amount of apoalanine aminotransferase is relatively constant over a wide range of enzyme activities.
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CITATION STYLE
Lustig, V. (1977). Activation of alanine aminotransferase in serum by pyridoxal phosphate. Clinical Chemistry, 23(2 I), 175–177. https://doi.org/10.1093/clinchem/23.2.175