Homer modulates NFAT-dependent signaling during muscle differentiation

Citations of this article
Mendeley users who have this article in their library.


While changes in intracellular calcium are well known to influence muscle contraction through excitation contraction coupling, little is understood of the calcium signaling events regulating gene expression through the calcineurin/NFAT pathway in muscle. Here, we demonstrate that Ca+2 released via the inositol trisphosphate receptor (IP3R) increases nuclear entry of NFAT in undifferentiated skeletal myoblasts, but the IP3R Ca+2 pool in differentiated myotubes promotes nuclear exit of NFAT despite a comparable quantitative change in [Ca+2]i. In contrast, Ca+2 released via ryanodine receptors (RYR) increases NFAT nuclear entry in myotubes. The scaffolding protein Homer, known to interact with both IP3R and RYR, is expressed as part of the myogenic differentiation program and enhances NFAT-dependent signaling by increasing RYR Ca+2 release. These results demonstrate that differentiated skeletal myotubes employ discrete pools of intracellular calcium to restrain (IP3R pool) or activate (RYR pool) NFAT-dependent signaling, in a manner distinct from undifferentiated myoblasts. The selective expression of Homer proteins contributes to these differentiation-dependent features of calcium signaling.




Stiber, J. A., Tabatabaei, N., Hawkins, A. F., Hawke, T., Worley, P. F., Williams, R. S., & Rosenberg, P. (2005). Homer modulates NFAT-dependent signaling during muscle differentiation. Developmental Biology, 287(2), 213–224. https://doi.org/10.1016/j.ydbio.2005.06.030

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free