Hepatoprotective Effect of Spondias pinnata in Isoniazid-Rifampicin-Induced Toxicity in Wistar rats

Abstract

Anti-tubercular (TB) drugs can cause oxidative stress, leading to hepatotoxicity. Plant extracts have antioxidant effects that can reduce oxidative stress. One plant with a high antioxidant capacity is Spondias pinnata. This study attempts to demonstrate that Spondias pinnata leaf extract, at doses of 200, 400, and 600 mg/kg bw (body weight), can decrease oxidative stress in rats treated with isoniazid-rifampicin. Five treatment groups, normal control (K0), negative control (K1), treatment 1 (P1), treatment 2 (P2), and treatment 3 (P3) were included in this experiment. Male Wistar strain (8–12 weeks old) and weighed 200–220 g totalling 35 were used in this study. K1, P1, P2, and P3 rats were given isoniazid-rifampicin for 28 days. Rats in groups P1, P2, and P3 received Spondias pinnata leaf extract for 35 days (starting 7 days before induction). The ELISA method was used to assess oxidative damage through 8-hydroxy-2'-deoxyguanosin (8-OHdG) levels in rat liver tissue. In the rats' liver result, isoniazid and rifampicin cause DNA oxidative stress, as seen by an increase in 8-OHdG levels in K1 relative to K0. In comparison to K1, the administration of 200 mg/kg (P1), 400 mg/kg (P2), and 600 mg/kg (P3) of Spondias pinnata leaf extract lowered the amount of 8-OHdG in the liver of rats induced by isoniazid-rifampicin. This study shows that Spondias pinnata exhibited hepatoprotective effects, reducing 8-OHdG levels in isoniazid-rifampicin-induced rats.