In order to understand the microbial inactivation mechanism of high-pressure carbonation (HPC), we examined the changes in the activity of the respiratory chain and DNA damage in Escherichia coli cells. HPC was performed under 1-6 MPa at 30°C for 1 min. The increase in CO2 pressure decreased the number of viable cells of E. coli, intracellular ATP, and intracellular NADH, and increased the number of apurinic/apyrimidinic sites. These results indicate that HPC has a detrimental effect on the functioning of the respiratory chain in E. coli and induces DNA damage, which could result in the death of the bacterial cells.
CITATION STYLE
Klangpetch, W., Noma, S., Igura, N., & Shimoda, M. (2013). Effects of high-pressure carbonation on intracellular ATP and NADH levels and DNA damage in Escherichia coli cells. Biocontrol Science, 18(4), 199–203. https://doi.org/10.4265/bio.18.199
Mendeley helps you to discover research relevant for your work.