Identification of mycobacterial antigens in human urine by use of immunoglobulin g isolated from sera of patients with active pulmonary tuberculosis

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Abstract

Point-of-care (POC) diagnostic testing of tuberculosis (TB) is a tremendous unmet need. In this study, four urinary mycobacterial antigens were identified through two independent approaches using IgG capture and immunodepletion methods. Among these, ModC was validated by a multiple reaction monitoring (MRM) method. As expected, the biomarkers elevated the clinical validity of TB diagnosis when combined with preexisting markers.

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Kim, S. H., Lee, N. E., Lee, J. S., Shin, J. H., Lee, J. Y., Ko, J. H., … Kima, Y. S. (2016). Identification of mycobacterial antigens in human urine by use of immunoglobulin g isolated from sera of patients with active pulmonary tuberculosis. Journal of Clinical Microbiology, 54(6), 1631–1637. https://doi.org/10.1128/JCM.00236-16

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