Microrna-24 regulates osteogenic differentiation via targeting T-cell factor-1

Abstract

MicroRNAs (miRNAs) have been reported to have diverse biological roles in regulating many biological processes, including osteogenic differentiation. In the present study, we identified that MiR-24 was a critical regulator during osteogenic differentiation. We found that overexpression of MiR-24 significantly inhibited osteogenic differentiation, which decreased alkaline phosphatase activity, matrix mineralization and the expression of osteogenic differentiation markers. In contrast, inhibition of MiR-24 exhibited an opposite effect. Furthermore, we delineated that MiR-24 regulates post-transcriptionals of T-cell factor-1 (Tcf-1) via targeting the 3'-untranslated region (UTR) of Tcf-1 mRNA. MiR-24 was further found to regulate the protein expression of Tcf-1 in the murine osteoprogenitors cells and bone mesenchymal stem cells. Additionally, the positive effect of MiR-24 suppression on osteoblast differentiation was apparently abrogated by Tcf-1 silencing. Taken together, our data suggest that MiR-24 participates in osteogenic differentiation by targeting and regulating Tcf-1 expression in osteoblastic cells.