Aberrant DNA hypermethylation-regulated long non-coding RNA RYR3-DT affects ferroptosis and progression of glioma cell through the CoQ10/FSP1 axis

Abstract

The RYR3-DT divergent transcript is a long noncoding RNA overexpressed in gliomas. DNA methylation is frequently perceived as a crucial process associated with epigenetic changes and among the preeminent mechanisms involved in gene inactivation. Ferroptosis is a recently discovered class of cell necrosis and death. Ferroptosis stimulation can potentially eliminate malignancies. RYR3-DT expression and its exact mechanism in glioma remain ambiguous. This investigation found that RYR3-DT was markedly elevated in gliomas. In-vitro functional assays, including Transwell assay, CCK-8, Western blot, and Annexin V assay, revealed that RYR3-DT silencing inhibits glioma cells growth, migration, and invasion, while stimulated cellular apoptosis. Western blot analysis and RNA sequencing proved that RYR3-DT regulated the ferroptosis CoQ10/FSP1 pathway, concluding that lncRNA RYR3-DT deletion might suppress glioma cell growth migrating and invading ability, and also increase cellular apoptosis via CoQ10/FSP1 pathway. Our research recommends lncRNA RYR3-DT is an expected target for future discoveries on glioma treatment.