Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease

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Abstract

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila. © 2013 by the Genetics Society of America.

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Gratz, S. J., Cummings, A. M., Nguyen, J. N., Hamm, D. C., Donohue, L. K., Harrison, M. M., … O’connor-Giles, K. M. (2013, August 1). Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics. https://doi.org/10.1534/genetics.113.152710

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