Discrimination of korean Rehmannia glutinosa from chinese Rehmannia glutinosa using sequence-characterized amplified region marker

Abstract

Rehmanniae Radix, from the roots of Rehmannia glutinosa Libosch has been used in traditional herb medicine for the treatment of fever and strengthening liver function, among others. Information on the phylogenetic relationship is very limited in the region of its cultivation. It is very important to know the information of the close relatives of R. glutinosa Libosch and R. glutinosa Libosch. f. hueichingensis Hsiao, R. glutinosa produced in Wen County, Meng County, Bo'ai County, Qinyang County in Henan province, China. In this study, we examined the polymorphism analysis of Rehmanniae Radix originated from both Korea and China to compare the difference at the genomic DNA level. Results revealed that ITS and rps16 region sequences of R. glutinosa in Korea and R. glutinosa in China were correspond, while randomly amplified polymorphic DNA analysis showed a difference in UBC 301 primer. The specific primer designed was amplified at 334 bp for R. glutinosa originated from China. This primer (HRgF and HRgR) would be used efficiently to distinguish R. glutinosa from different sources. © 2012 The Korean Society for Applied Biological Chemistry.