AMPLIFIKASI GEN CAPSAICIN SEBAGAI GEN PENANDA PADA MIKROBA ENDOFIT BUAH CABE RAWIT

Abstract

Endophytic bacteria are microorganisms that live in plant tissues. Endophytic bacteria are known to have genetic similarities with their host plants. Amplification of the capsaicin coding gene using endophytic bacterial DNA is one of the method to study the genetic similarity pattern of endophytic bacterial groups with chili pepper. The purpose of this study was to amplify DNA of endophytic bacteria from chili pepper using the capsaicin gene to see the potential endophytic bacteria producing capsaicin. Furthermore is to study the pattern of genetic similarity with the band on the amplicon. The method of isolation of endophytic bacteria using the pour plate method then subcultured the pure isolates by using the streak plate method. The DNA was extracted from pure isolates of endophytic bacteria using the phenol-chloroform method and DNA purification kit. The DNA of endophytic bacteria was amplified using specific primers. The results showed that four endophytic bacterial isolates of chili pepper were BE1, BE2, BE3, and BE4. Then performed amplification using a specific primer. The amplification results did not show any amplicons, so further optimization was carried out using touchdown PCR. After optimization, there was amplification between the primers and the template DNA which became the target DNA. From the amplification results, it was found that the target DNA was not attached to a specific primer for the capsaicin gene.