P-coumaric acid is a nonflavonoid phenolic acid and is a major constituent of the species Cynodon dactylon Linn. (Pers.). In this study isolation of P-coumaric acid was achieved by preparative TLC and the compound thus isolated was characterised by UV, mass, and H1 NMR spectral analysis. An isocratic RP-HPLC method was developed for the estimation of P-coumaric acid from methanolic extracts of durva grass. The chromatographic separations were achieved by RP-C18 column (250 mm × 4.6 mm, 5), Shimadzu LC-20AT Prominence liquid chromatograph, and a mobile phase composed of water: methanol: glacial acetic acid (65: 34: 1 v/v). The flow rate was 1.0 mL/min and the analyses of column effluents were performed using UV-visible detector at 310 nm. Retention time of P-coumaric acid was found to be 6.617 min. This method has obeyed linearity over the concentration range of 2-10 g/mL and the regression coefficient obtained from linearity plot for P-coumaric acid was found to be 0.999. RP-HPLC method was validated in pursuance of ICH guidelines.
Karthikeyan, R., Devadasu, C., & Srinivasa Babu, P. (2015). Isolation, characterization, and RP-HPLC estimation of P-coumaric acid from methanolic extract of durva grass (Cynodon dactylon Linn.) (Pers.). International Journal of Analytical Chemistry, 2015. https://doi.org/10.1155/2015/201386