Rapid Determination of 3 Components With Different Polarities in Medicinal Mushrooms by Multistep Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography Analysis

Abstract

In this study, a multistep matrix solid-phase dispersion (MSPD) combining with a high-performance liquid chromatography method was developed for assaying 3 components of different polarities (mannitol, adenosine, and ergosterol) from mushroom samples. MSPD extraction was carried out using 1 g octadecyl-bonded silica as the sorbent material, 9% methanol, 20% methanol, and 100% methanol as eluting solvents for the elution of mannitol, adenosine, and ergosterol, respectively. Mannitol was separated on an NH2P-50 4E column and detected using an evaporative light scattering detector. Adenosine and ergosterol were separated on a Poroshell 120 SB-C18 column and measured at 260 nm and 283 nm, respectively. The developed method showed good linearity (R ≥ 0.9986) within the test range. The relative SD (RSD) of precisions were less than 1.4%, and the recoveries were 95.6%-97.0% (RSD ≤3.0%). Compared with the reported methods, the developed procedure could rapidly prepare components with different polarities (mannitol, adenosine, and ergosterol) from medical mushroom samples with less organic solvent and sample. The method is rapid and eco-friendly, which is helpful to improve the quality evaluation of medicinal mushrooms.