Objective: To improve the efficacy of an IVF-ET program for unstimulated patients with polycystic ovary syndrome (PCOS) with the use of culture for oocyte maturation. Design: Prospective studies with the comparison of different ET procedures from March 1995 through February 1998. Setting: University-affiliated hospital. Patient(s): Ninety-four cycles in 64 consenting patients with PCOS. Intervention(s): Immature oocytes were retrieved from unstimulated patients with PCOS and subsequently cultured and fertilized in vitro. Zygote intrafallopian transfer (ZIFT), uterine ET, or a combined approach of ZIFT + uterine ET was subsequently performed. Main Outcome Measure(s): Laboratory and clinical data. Result(s): Among 1,280 immature oocytes (13.6 ± 7.5 oocytes per patient) retrieved, 89% (1,139) were morphologically normal, and 62.2% (708/1,139) of the normal oocytes matured in vitro after culture for 48 hours. When intracytoplasmic sperm injection was performed, 68% (481/708) developed to the normal pronuclear stage, and 88.1% of the embryos cocultured with Vero cells (266/302) cleaved. Eighty-five ET cycles were conducted and pregnancy was established in 23 cycles (27.1%), which consisted of 8 after uterine ET and 15 after a combined approach. Seventeen patients delivered 20 normal infants. Conclusion(s): The IVF-ET method using no ovarian stimulation followed by in vitro maturation culture can be a feasible assisted reproductive technology for treatment of PCOS with various complications. (C)2000 by American Society for Reproductive Medicine.
Cha, K. Y., Han, S. Y., Chung, H. M., Choi, D. H., Lim, J. M., Lee, W. S., … Yoon, T. K. (2000). Pregnancies and deliveries after in vitro maturation culture followed by in vitro fertilization and embryo transfer without stimulation in women with polycystic ovary syndrome. Fertility and Sterility, 73(5), 978–983. https://doi.org/10.1016/S0015-0282(00)00422-2