Caracterización de una fuente celular bovina multipotente y su potencial aplicación como modelo para la evaluación de biomateriales

Abstract

Animal models have been extensively applied for research on developmental biology and for cell lineages characterization, especially with higher animals believed to be more related to human cell biology. This work reports the establishment of a fetal bovine chondrogenic cell line and demonstrates its multipotentiality through the qualitative evaluation of its chondrogenic and osteogenic potential. This cell source is capable of matrix mineralization upon cell induction with medium supplementation and under the proper microenvironment can retain its chondrocytic phenotype. These suggest the potential application of this cell line for biomaterial evaluation, particularly bone tissue engineering and the characterization of osteoinductivity. It constitutes an additional source to the use of human bone marrow stem cells for the biological evaluation of biomaterials. The advantages presented for the bovine model include reduced ethical issues, increased availability and that the fetal nature of the tissue supposes a greater multipotentiality compared to human adult multipotent cells. © Springer-Verlag Berlin Heidelberg 2007.