Practical Handbook of the Biology and Molecular Diversity of Trichoderma Species from Tropical Regions

  • Siddiquee S
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Abstract

This book analyzes the right pathway to solve the controversial identifications of some Trichoderma species on the basis of sampling procedures, slide culture techniques, macroscopic and microscopic analysis, and molecular tools. Most species of the genus Trichoderma grow rapidly in artificial culture and produce large numbers of small green or white conidia from conidiogenous cells located at the ends of conidiophores. The morphological characters are reported to be variable to a certain degree in their color, shape of conidia, conidiophore, pustules and phialade. These characteristics allow a comparatively easy means of identification of Trichoderma as a genus, but the species concept is difficult to deduce and there is considerable confusion over the application of specific names. This work provides an essential link between data and taxa as a means to verify the taxonomic characters of the strains sequenced, and mac roscopic and microscopic characteristics. Otherwise, a species level identification study cannot be corrected or uncorrected and the user has to rely on the person making the misidentification. . Preface; Contents; About the Author; Chapter 1: The Basic Concept of Microbiology; 1.1 Aseptic Technique and Safety; 1.2 Good Microbiological Laboratory Practices (GMLP); 1.3 Risk Assessment; 1.4 Spillage Management; 1.4.1 Spills; 1.4.2 Broken Glass; 1.4.3 Splashes on Clothing and the Skin; 1.5 Resources; 1.5.1 Equipment (Source: ASE 2001); 1.5.2 Apparatus (Source: ASE 2001); 1.5.3 Materials (Source: ASE 2001); 1.6 Flaming the Needle/Loop; 1.7 Flaming the Neck of Bottles/Test Tubes; 1.8 Media; 1.8.1 Preparation of Culture Media; 1.8.2 Pouring a Petri Plate; 1.8.3 Agar Slants 1.8.4 Storage of Prepared Media1.9 Disinfectants; 1.10 Incubation; 1.11 Accidents; 1.12 Emergency Preparation and Response; References; Chapter 2: Collection and Processing for Trichoderma Specimen; 2.1 Introduction; 2.2 Media for Isolating of Trichoderma; 2.2.1 Trichoderma Selective Media; 2.2.2 T. harzianum-Selective Medium (THSM); 2.2.3 Rose Bengal Agar (RBA); 2.3 Materials; 2.4 Methods; 2.4.1 Sample Collection Procedures; 2.4.2 Isolation of Trichoderma Colony; 2.4.3 Sample Dilution; 2.4.4 Culturing Plates; 2.4.5 Preparation of Slant Media; 2.4.6 Slant Culture 2.5 Identification of Trichoderma Isolates2.6 Pure Culture of Trichoderma Isolates; 2.7 Direct Culture Methods; 2.8 Storage of Trichoderma; 2.8.1 Storage in Water; 2.8.2 Cold Temperature; 2.8.3 Under Mineral Oil; 2.8.4 Freeze Drying or Lyophilization; 2.8.5 Low-Temperature Storage; References; Chapter 3: Slide Culturing of Trichoderma Isolates; 3.1 Introduction; 3.2 Materials; 3.3 Procedure; 3.3.1 Slide Culture Technique I; 3.3.2 Slide Culture Technique II; Chapter 4: Morphology-Based Characterization of Trichoderma Species; 4.1 Introduction 4.2 Definitions and Taxonomic Value of Morphological Traits4.2.1 Teleomorph; 4.2.2 Cultures; 4.2.3 Growth Media; 4.2.4 Growth Plates/Colonies; 4.2.5 Growth Plates/Conidiation; 4.2.6 Conidial Heads, Wet Versus Dry; 4.2.7 Conidiophores/Trees, Regular; 4.2.8 Conidiophore Terminology (Source: Jaklitsch 2009); 4.2.9 Effuse Conidiation (Synanamorphs); 4.2.10 Pustules; 4.2.11 Shrubs, Granules; 4.2.12 Tufts, Also Called "Fluffy Tufts"; 4.3 Macroscopic Analysis; 4.3.1 Trichoderma asperellum; 4.3.2 Trichoderma harzianum; 4.3.3 Trichoderma hamatum; 4.3.4 Trichoderma reesei 4.3.5 Trichoderma longibrachiatum4.3.6 Trichoderma spirale; 4.3.7 Trichoderma brevicompactum; 4.3.8 Trichoderma erinaceum; 4.3.9 Trichoderma koningiopis; 4.3.10 Trichoderma melanomagna; 4.3.11 Trichoderma viride; 4.3.12 Trichoderma theobromicola; References; Chapter 5: Molecular Characteristics of Trichoderma Strains; 5.1 Molecular Analysis; 5.2 DNA Extraction and Purification; 5.3 Obtaining Materials from Which to Extract DNA; 5.3.1 Trichoderma Culture; 5.3.2 Trichoderma Liquid Culture; 5.4 DNA Extraction Protocols; 5.4.1 Phenol-Chloroform Method

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Siddiquee, S. S. S. (2017). Practical Handbook of the Biology and Molecular Diversity of Trichoderma Species from Tropical Regions (p. 102). https://doi.org/10.1007/978-3-319-64946-7

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