myo -Inositol Synthesis from [1- 3 H]Glucose in Phaseolus vulgaris L. during Early Stages of Germination

Abstract

11-3Hjglucose did occur during the first 72 hours of germination. More than 90% of the labeled, free myo-inositol was present in the cotyledons. The 3H percentage in trifluoroacetic acid-soluble arabinose residues of cell wall polysaccharides from 72-hour-old bean hypocotyls was only half of their mole percentage. On the other hand, 3H percentages in hexose residues were higher than their mole percentages. The results suggest that myo-inositol is synthesized from reserve sugars during the very early stages of germination, and that the newly synthesized myo-inositol, as well as that stored in cotyledons, can be used for the construction of new hypocotyl and root cell wall polysaccharides after conversion into uronic acids and pentoses via the myo-inositol oxidation pathway. ditions to bean (Phaseolus vulgaris L.) seeds. After 72 and 96 hours of feeding, the 3H was located in uronic acid and pentose residues as well as hexose residues of cell wall polysaccharides in growing hypocotyl and root. Free myo-inositol present in cotyledons, hypocotyl, and root also contained 3H, showing that de novo synthesis of myo-inositol from Radiolabeled D-11-3Hjglucose was fed by imbibition under sterile con- acid and pentose residues in bean cell wall polysaccharides formed during very early stages (72 and 96 h imbibition treat- ment) of germination. We also report the synthesis of MI from D-[ I-3H]glucose. MATERIALS AND METHODS early stages of germination, we supplied i-[ 1-3H]gluc