In vitroCytotoxic Activity of a Sulphated Polysaccharide Ulvan against Human Breast and Glioblastoma Cell line

Abstract

This study was designed to evaluate the hemolysis, anti-inflammatoryand cytotoxicityactivity of sulphated polysaccharideulvan extracted from greenseaweed Ulvalactuca(UL).Ulvan was extracted and characterized with UV-Visible Spectroscopy, Fourier transform infraredspectroscopy (FTIR) and Scanning electron microscopy(SEM).Total uronic acid and sulphate content were found to be 19.38% w/w and 22.53% w/w.The biocompatibilityof the sulphated polysaccharide ulvan wasevaluatedviahemolysis, its interaction with erythrocyte structure and anti-inflammatory studies.Further,thepolysaccharide was treated for cellular toxicityagainsthuman breast cancer(MCF-7)and human glioblastoma cancer (U87) cell linesusing the 3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assaymethod.This study indicates that the sulphated polysaccharide ulvanwas more suitable for biomedical applications with promising chemo preventive and chemotherapeutic activity.©2022iGlobal Research and PublishingFoundation. All rights reserved.Cite this article as:Sonia, K.; Meena, K.S.; Alamelu Bai, S.In vitro Cytotoxic Activity of a Sulphated Polysaccharide Ulvan against Human Breast and Glioblastoma Cell line.Indo Global J. Pharm. Sci., 2022; 12:122-127. DOI:http://doi.org/10.35652/IGJPS.2022.12012.