Drosophila Jing is part of the Breathless fibroblast growth factor receptor positive feedback loop

Abstract

In the developing Drosophila trachea, extensive cell migration lays the foundation for an elaborate network of tubules to form. This process is controlled by the Drosophila fibroblast growth factor receptor, known as Breathless (Btl), whose expression is activated by the Trachealess (Trh) and Tango (Tgo) basic helix-loop-helix (bHLH)-PAS transcription factors. We previously identified the jing zinc finger transcription factor as a gene sensitive to the dosage of bHLH-PAS transcriptional activity and showed that its mutations interact genetically with those of trh and btl. Here, we demonstrate that jing is required for btl expression in the branching trachea and dominantly interacts with known regulators of btl expression, including the ETS and POU transcription factors, pointed, and drifter/ventral veinless, respectively. Furthermore, the zinc fingercontaining C-terminus of Jing associates with a btl tracheal enhancer in a Trh/Tgo-dependent manner in chromatin immunoprecipitation assays in vitro and interferes with btl in vitro and in vivo. Together, our results support a model by which Jing/Trh/Tgo complexes regulate btl transcript levels during primary tracheal branching. © Springer-Verlag 2010.