Effects of DNA supercoiling and topoisomerases on the expression of genes coding for F1651, a P-like fimbriae

8Citations
Citations of this article
11Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Pathogenic Escherichia coli 4787 (O115:KV165) causes septicemia in pigs and expresses the fimbriae F1651 encoded by the foo operon that belongs to the P fimbrial family. fooI and fooB, encoding specific foo regulators, are divergently transcribed; their intergenic region is responsible for the regulation of foo expression. The role of global and local supercoiling (transcription-induced supercoiling within the intergenic region) on the regulation of foo expression was investigated. Expression of fooB was significantly altered when global negative supercoiling was reduced by a mutation that decreases DNA gyrase activity. Deletion of the topA gene, encoding for topoisomerase I that relaxes local negative supercoiling, further reduced fooB expression. This suggests that both global and local supercoiling can significantly affect fooB expression. Moreover, FooI, a positive regulator of fooB expression, has no effect on fooB expression in the topA null mutant. This study showed that divergent transcription from a strong promoter can significantly enhance fooB expression and compensate for the absence of FooI in a wild-type strain. © 2007 Federation of European Microbiological Societies.

Author supplied keywords

Cite

CITATION STYLE

APA

Tessier, M. C., Graveline, R., Crost, C., Desabrais, J. A., Martin, C., Drolet, M., & Harel, J. (2007). Effects of DNA supercoiling and topoisomerases on the expression of genes coding for F1651, a P-like fimbriae. FEMS Microbiology Letters, 277(1), 28–36. https://doi.org/10.1111/j.1574-6968.2007.00919.x

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free