The Study of Tyrosinase and Antioxidant Activity of Xylocarpus Granatum Koenig Seed Kernel Extract toward Evidence Based Indigenous Knowledge from Togean Archipelago, Indonesia

Abstract

The tyrosinase inhibitory activity and antioxidant activity of three (3) extracts (methanol, chloroform,and n-hexane) from seed kernel of Xylocarpus granatum Koenig was tested. The tyrosinase inhibitory activity and antioxidant activity was estimated as IC50 values and the content of total phenolics in extracts was calculated as gallic acid equivalent (GAE). The best result of tyrosinase inhibitory ability was obtained in methanol extract in monophenolase activity (IC50 =323.11 ± 9.0 μg/mL that correlated significantly with the total of phenolic content (R2 =0.98, Y= -12.27x + 471.73) and the antioxidant activity have IC50 = 10.61 ± 0.6 μg/mL. We indicate that this methanolic extract of X. granatum seed kernel posseses potency as tyrosinase inhibitor and antioxidant agent. This result has already shown that the indigenous knowledge in utilization of X. granatum seed kernel for lightening skin has been evident.