Polymerase Chain Reaction in the Diagnosis of Spontaneous Leptospirosis in Bovines

Abstract

Accurate and prompt diagnosis of leptospiral infection in bovine is difficult due tolimitation of current procedures. The present study describes the application of polymerasechain reaction (PCR) for detection of leptospiral deoxyribonucleic acid (DNA) in bloodand urine samples in the spontaneous cases of bovine leptospirosis. In most of studiesleptospires DNA detected by PCR in experimentally induced infection so in present studytrying to evaluate this assay in naturally infected cattle and buffalo. A total of 120 bloodand 304 urine samples were collected from cattle and buffaloes. These samples weresubjected to PCR using two sets of primers i.e. G1/G2 and LipL32 and it revealed that9/120 blood and 33/304 urine samples were found to be positive. The results suggestedthat PCR could be an excellent approach for diagnosis of Leptospirosis in field samplesalso. The specificity of primers was checked by using reference pathogenic serovar ofLeptospira (11) (PDF) Polymerase Chain Reaction in the Diagnosis of Spontaneous Leptospirosis in Bovines. Available from: https://www.researchgate.net/publication/321879363_Polymerase_Chain_Reaction_in_the_Diagnosis_of_Spontaneous_Leptospirosis_in_Bovines [accessed Oct 16 2022].