Synergetic effect of rhizosphere bacteria isolates and composted manure on fusarium wilt disease of tomato plants

Abstract

Composts and bio-fertilizers are the most important elements for agricultural development activities. In agricultural both practices are prominently improves plant growth. Besides, Composts and bio-fertilizers suppress plant disease, presence of a pathogen and a susceptible host in the environment. Plant disease suppression is the direct result activity of consortia antagonistic microorganisms and composted manure. Isolation and identification of the soil borne fungal pathogens and rhizosphere bacteria were done in vitro based on their morphological and biochemical examination; to evaluate effects of rhizosphere bacteria isolates and composted manure on Fusarium wilt of tomato/Lycopersicon esculentum/plants disease. Additionally, antagonism experiment likewise hydrogen cyanide test and plant growth promotion test (phosphate solubilization, starch hydrolysis and screening of Indole Acetic Acid (IAA)) productions were carrying out. Under greenhouse condition the disease severity, disease incidence and measurement of growth parameters were correlated using two rhizosphere isolates (RhB-7 = Rhizobacteria Bacillus spp., RhP-12 = Rhizobacteria Pseudomonas spp.) and two different percentages of composted manures (10 and 20%) including their combination. The result revealed 100% disease incidence and severity in control experiment and 29-100% efficacy in combined treatment. Furthermore, the combination of RhB-7, RhB-12 and 20% of composted manure were the most efficient against Fusarium wilt disease of tomato than the rest treatments. Through, rhizosphere bacteria isolates and composted manure it is possible to control tomato/Lycopersicon esculentum/plant wilt which is caused by the fungal called Fusarium spp. and increase the soil fertility and the product yields.