This chapter presents the different transformation methods used for Trichoderma genetic transformation, including Agrobacterium tumefaciens [Rhizobium radiobacter]-mediated transformation, polyethylene glycol-mediated protoplast transformation, biolistic transformation, and electroporation. The advantages and disadvantages of these strategies are discussed. The use of genetic markers and reporter genes, gene targeting, site-specific recombination systems, and post-transcriptional gene silencing with RNA interference in the various stages of Trichoderma genetic engineering is discussed.
Steiger, M. G., Blumhoff, M. L., Mattanovich, D., & Sauer, M. (2013). Biochemistry of microbial itaconic acid production. Frontiers in Microbiology. Frontiers Research Foundation. https://doi.org/10.3389/fmicb.2013.00023