Laccase and borate-fructose complex were investigated by coincidence in a solid-state fermentation of Edenia sp. TS-76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34-fold purification and 32% yield. Fructose had no significant effect on laccase activity, whereas borate reduced laccase activity by 60-90%; conversely, the borate-fructose complex increased laccase activity by nearly fourfold at pH 7.5. The complex caused a shift in the optimal pH for laccase from 5.0 to 7.5 and served as a highly efficient mediator. Borate complexed with fructose provides an alternative, time-saving, and specific method for serum fructose determination.
Cheng, C. Y., Liao, C. I., & Lin, S. F. (2015). Borate-fructose complex: A novel mediator for laccase and its new function for fructose determination. FEBS Letters, 589(20), 3107–3112. https://doi.org/10.1016/j.febslet.2015.08.032