Glycoproteins influence a broad spectrum of biological processes including cell-cell interaction, host-pathogen interaction, or protection of proteins against proteolytic degradation. The analysis of their glyco-structures and concentration levels are increasingly important in diagnosis and proteomics. Boronic acids can covalently react with cis-diols in the oligosaccharide chains of glycoproteins to form five- or six-membered cyclic esters. Based on this interaction, boronic acid-based ligands and materials have attracted much attention in both chemistry and biology as the recognition motif for enrichment and chemo/biosensing of glycoproteins in recent years. In this work, we reviewed the progress in the separation, immobilization and detection of glycoproteins with boronic acid-functionalized materials and addressed its application in sensing. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Wang, X., Xia, N., & Liu, L. (2013, October 17). Boronic acid-based approach for separation and immobilization of glycoproteins and its application in sensing. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms141020890