Identification of insertion sequence from a γ-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26

Abstract

Tn5-derived mutants of the γ-hexachlorocyclohexane-degrading bacterium Sphingomonas paucimobilis UT26 were genetically characterized, and an endogenous insertion sequence (IS) which belongs to the IS1380 family was identified. The IS, earned ISsp1, existed as multi copies in UT26, and its transposition appeared to be activated during the process of Tn5-mutagenesis. It was found that transposon mutagenesis can cause endogenous mutations.