Nucleotide sequence of a cellulase gene of Bacillus subtilis

Abstract

The nucleotide sequence of an endolytic cellulase gene of Bacillus subtilis was determined and compared with the NH2‐terminal amino acid sequence of the purified enzyme. The mature protein appeared to be extended by a signal sequence of 36 amino acids. The putative AUG initiation codon was preceded by a σ43‐type promoter of B. subtilis and an AAGGAGG sequence, typical of procaryotic ribosomal binding sites. Partial homology of amino acid sequences was dound between B. subtilis cellulase and an alkalophilic Bacillus cellulase. Copyright © 1987, Wiley Blackwell. All rights reserved