Luteolin ameliorates colistin-induced nephrotoxicity in the rat models

Abstract

Introduction and aim: To study the protective, preventive effect of luteolin from colistin-induced nephrotoxicity. Material and method: Four different treatment options were tested on rats: colistin, luteolin, and a combination of colistin and luteolin, intraperitoneally as two doses a day, for seven days. Another group of rats were used as the control and treated with sterile saline. Serum creatinine levels were measured before and after treatment. Histological changes and colistin-induced apoptosis (Insitu BrdU-red DNA Fragmentation Assay Kit) of the renal tissues were examined after the scarification procedure. Results: In the Colistin Group, post-treatment creatinine levels were statistically higher than the pretreatment levels (p =.001). In the remaining groups, no significant changes were observed. Cells that undergo apoptosis were counted and it was shown that all groups except the colistin-treated group had a similar number of apoptotic cells, whereas the colistin-treated group had statistically higher number of apoptotic cells compared to other groups (p=.0001). Renal histological damage was also measured and the score of the colistin treated group was higher as compared to other groups. Conclusion: The results obtained from this study demonstrated us that luteolin was capable of preventing colistin-induced nephrotoxicity and that this effect was significant at histopathological level.