Molecular Identification of Virulence Genes in Pseudomonas aeruginosa Isolated from Human and Animal Samples and Their Antibiotic Resistance Pattern

Abstract

Background and Aims: A major problem in the treatment of Pseudomonas aeruginosa infections is the emergence of strains with multiple resistances (MDR). The aim of this study was to identify virulence genes lasB, toxA, algD, exos in P. aeruginosa isolated from human and animal using Multiplex-PCR method and determination of antibiotic resistance. Materials and Methods: This cross-sectional study was performed on the 120 non-repetitive samples, including, 60 clinical samples of human and 60 animal samples collected from Tehran, Iran. Antibiotic susceptibility test was performed by disk diffusion test. The multiplex - PCR method was performed to identify various virulence genes. Results & Conclusion: The highest resistance rate was related to amoxicillin and amikacin in the both types of samples. lasB and exoA genes were the most prevalent virulence determinants in the human and animals samples, respectively. The results of antibiotic susceptibility test showed that the resistance in human strains was far higher than animal and this could be the result of arbitrary administration of the drug. The lasB gene in human specimens and the exoA gene in animals play an important role in the development of the disease.