Isolasi dan Karakterisasi Selulase dari Trichoderma Viride dan Rhizopus Spp dengan Substrat Jerami Padi

Abstract

The objectives of this study were to isolated and produce cellulose from molds and to characterize the enzyme. The strain of mold isolated from soil, namely Rhizopus spp and one cultured mold, Trichoderma viride, was used to produce the enzymes. Medium for enzyme production consisted of NH4NO3, KCL, FeSO47H2O, MgSO47H2O, CuSO42H2O and rice straw as a sole source of carbon (pH = 5,45). Culture was done at 28oC for 5,8,11 and 14 days with substrat concentration 1,5% for T. viride culture. However the optimum production of cellulose for Rhizopus spp culture were three days shorter than culture of T. viride with substrat concentration of 1,5 and 1% respectively. Temperature and pH optimal activity of cellulose were as follow ; cellulose from T. viride at temperature 60oC and pH 5 and cellulose from Rhizopus spp at temperature 50oC and pH 5. While temperature and pH stability of celluloses were as follow : cellulose from T. viride at temperature 30-80oC and pH 3-7 and cellulose from Rhizpous spp at temperature 30-80oC and pH 3-6. It was concluded that molds can grow on rice straw as a sole source of carbon produce celluloses.